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TIGR Maize TA
Maize Repeat db
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AZM5
TIGR maize BACs
Gene annotation

Cell Genomics
Fluorescent-tagged subcellular markers
 

TIGR BAC sequencing

     In the second phase of the Consortium for Maize Genomics project, we have sequenced 287 BACs from a combination of the maize BAC libraries CHORI-201 B73 (ZMMBBc) constructed by Pieter J. de Jong and NSF B73 (ZMMBBb) constructed by Jeff Tomkins.

Summary

  1. BACs were end-sequenced and fingerprinted for identity confirmation.
  2. BAC Shotgun libraries contained an insert size of 6-8 kb.
  3. Paired-end reads for approximately 1,000 clones were produced, which provided approximately 5 to 7.5 fold sequence coverage.
  4. Sequence reads and assembled BAC sequences were deposited at NCBI Trace archive and GenBank respectively once available.
  5. Assembled BACs were annotated using an improved automated TIGR annotation pipeline optimized for maize.