| Characterizing Sub-Cellular Compartments
in Maize Using Fluorescent Protein Tagged Lines |
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What's new
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| June 2009 |
A book chapter which describes detailed methods and protocols by Mohanty et al. (Download PDF).
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| Feb 2009 |
First comprehensive set of maize fluorescent protein tagged lines published by Mohanty et al. (Download PDF). Find out how to order
seeds or
clones. |
| May 2008 |
Updates on genetics workshop with Little Big Horn College, MT (May 12-16, 2008) posted
here. |
| Mar 2008 |
Seeds available from tagged lines. Click
here.
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| Jul 2007 |
Updates on genetics workshop with Little Big Horn College, MT (July 23-30, 2007) posted
here. |
| May 2007 |
Seeds available by contacting
Dave Jackson or
Anne Sylvester.
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| Apr 2006 |
You can now submit a gene for fluorescent tagging.
The gene submission form is available here.
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| Jul 2005 |
Project receives fundings from the National Science Foundation to characterize
subcellular localization of maize proteins
NSF Award #0501862.
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| Gene construct status | Total=59 |
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| Tr - Construct tested by transient essay | 1 |
| C - Construct submitted for maize transformation | 5 |
| P - Transformed maize plant obtained | 18 |
| E - Expression of tagged protein verified | 35 |
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| Expression images and movies | Total=146 |
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Examples of tagged maize genes
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| Tagged genes | Predicted localization | Status |
| Tubulin-alpha | microtubules | E |
| Abphyl1 | nuclear | E |
| Fea2 | unknown | E |
| Histone H1B | chromatin | E |
| Tonoplast intrinsic protein TIP1 | vacuolar membrane | E |
| Alpha-zein | protein bodies, ER | E |
| Ramosa 3 | unknown | E |
| Pinformed1 | plasma membrane, vesicles | E |
| Warty (Rab2A1) | vesicles, ER or Golgi | E |
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Number of visits since Apr 2006 : 08058
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